[1]黄曹兴,房伶晏,赖晨欢,等.不同预处理对毛竹木质素抗氧化性的影响[J].林业工程学报,2018,3(03):73-80.[doi:10.13360/j.Issn.2096-1359.2018.03.013]
 HUANG Caoxing,FANG Lingyan,LAI Chenhuan,et al.Effects of different pretreatments on antioxidant activities of Moso bamboo lignin[J].Journal of Forestry Engineering,2018,3(03):73-80.[doi:10.13360/j.Issn.2096-1359.2018.03.013]
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不同预处理对毛竹木质素抗氧化性的影响()
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《林业工程学报》[ISSN:1001-8081/CN:32-1160/S]

卷:
3
期数:
2018年03期
页码:
73-80
栏目:
生物质能源与材料
出版日期:
2018-05-15

文章信息/Info

Title:
Effects of different pretreatments on antioxidant activities of Moso bamboo lignin
文章编号:
2096-1359(2018)03-0073-08
作者:
黄曹兴123房伶晏3赖晨欢13唐硕13梁辰2李鑫13勇强13*
1.南京林业大学林业资源高效加工利用协同创新中心,南京 210037;
2.广西清洁化制浆造纸与污染控制重点实验室,南宁 530004;
3.南京林业大学化学工程学院,南京 210037
Author(s):
HUANG Caoxing123 FANG Lingyan3 LAI Chenhuan13 TANG Shuo13 LIANG Chen2 LI Xin13 YONG Qiang13*
1.Jiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Products, Nanjing Forestry University, Nanjing 210037, China;
2.Guangxi Key Laboratory of Clean Pulp & Papermaking and Pollution Control, Nanning 530004, China;
3.College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
关键词:
毛竹木质素 预处理 核磁共振技术 官能团 抗氧化性
Keywords:
Moso bamboo lignin pretreatment nuclear magnetic resonance functional groups antioxidant
分类号:
TQ358
DOI:
10.13360/j.Issn.2096-1359.2018.03.013
文献标志码:
A
摘要:
采用核磁共振技术(2D-HSQC NMR和定量31P NMR)分析毛竹木质素经稀硫酸法和硫酸盐法预处理后官能团含量和结构单元类型比例变化,并对预处理前后木质素抗氧化性能(1,1-二苯基-2-三硝基苯肼(DPPH)和超氧阴离子自由基清除能力)进行评价。核磁共振分析结果表明,毛竹木质素(MWLr)经稀硫酸法和硫酸盐法预处理(MWLa和KL),紫丁香基单元摩尔含量比例从50.6%提高至68.8%和72.2%,相连接的麦黄酮结构发生降解,脂肪族羟基、酚羟基和羧基质量分数均增加。抗氧化性结果表明,MWLr,MWLa和KL对DPPH自由基最大清除能力为79.59%,75.57%和77.69%,对超氧阴离子自由基最大清除能力为81.15%,47.27%和74.53%。稀酸和硫酸盐法预处理降低毛竹原本木质素抗氧化能力可能是由于木质素结构中麦黄酮被降解和紫丁香基结构质量分数比例增加导致。
Abstract:
In this study, lignins in Moso bamboo and acid-pretreated bamboo were isolated according to the method developed by Bjorkman, termed as MWLr and MWLa, respectively. Lignin in black liquor of pretreated bamboo by kraft pulping was precipitated by acid and termed as KL. Then, the obtained lignins(MWLr, MWLa, and KL)were characterized by 2D heteronuclear single quantum correlation nuclear magnetic resonance(2D-HSQC NMR)and quantitative 31P NMR to analyze the effects of different pretreatments on the functional groups and types of lignin units in the resulting lignin. Meanwhile, the performance of above obtained lignins on the scavenging of superoxide anion free radical and 1,1-diphenyl-2-picryl-hydrazyl(DPPH)radical were investigated. Results from the NMR analysis showed that the percentage of syringyl units in bamboo lignin increased from 50.6% to 68.8% and 72.2% after it was pretreated by acid and kraft pulping, respectively. In addition, the pretreatments resulted in degradation of tricin and increase in the amounts of alphatic hydroxyl groups, phenolic hydroxyl groups, and carboxylic acid groups in obtained lignin samples. It showed that the capabilities of MWLr, MWLa, and KL to scavenge DPPH radical were up to 79.59%, 75.57%, and 77.69%, respectively. For superoxide anion free radical, the highest scavenging rates were 81.15%, 47.27%, and 74.53% that achieved by MWLr, MWLa, and KL, respectively. These results indicated that the antioxidant activity of the obtained lignin decreased after it was pretreated by acid or kraft pulping process. The probable reasons for this result might be due to the degradation of tricin in lignin and the increased percentage of syringyl units in the lignin.

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备注/Memo

备注/Memo:
收稿日期:2017-10-13 修回日期:2017-12-12
基金项目:国家自然科学基金面上项目(31570561); 国家自然科学基金青年项目(31600463); 广西清洁化制浆造纸与污染控制重点实验室开放基金(KF201725); 江苏高校优势学科建设工程资助项目(PAPD)。
作者简介:黄曹兴,男,讲师,研究方向为生物质资源生物降解与转化。通信作者:勇强,男,教授。E-mail: swhx@njfu.com.cn
更新日期/Last Update: 2018-05-15